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Ion mobility now showing and not able to export

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Ion mobility now showing and not able to export laura corveleyn  2024-10-18 02:57
 

Hi Team,

I am looking at diaPASEF data in Skyline and want to include IM values in the export report. However, the IM values all say "NA" and when I want to show IM on the chromatogram (by right clicking) it doesn't show anything. But when I double click the peak I can see the mobilogram so the ion mobility data is definitely read in correctly. How can I fix this?

Best,

Laura
 
 
Brian Pratt responded:  2024-10-18 07:29

Hi Laura,

When you look at the ion mobility heatmap, do you see a horizontal purple band indicating the IM range that was used in extracting the chromatogram? If not, you've not yet assigned a mobility value to your precursors, which is why nothing shows in the report.

If that's not the case, I'd be happy to look at your data (use Skyline's File > Share menu item, and include at least one of the mass spec files).

Thanks for using the Skyline support board,

Brian Pratt