Trouble with Target Quantification and Integration in Skyline for PRM Data | afshari1 | 2024-09-13 14:44 | |||||||||||||||||||||||||||||||||||
Hello, I am analyzing my PRM results using Skyline and encountering an issue with quantifying certain targets. Specifically, in the attached file, there is a peak at 69.x minutes in the bottom panel, which corresponds to one of my targets. The MS/MS data from Thermo Fisher software confirms this match. However, when I import the same RAW file into Skyline, this target is not being quantified or integrated. Could you please help me understand why this might be happening and how I can resolve this issue? |
|||||||||||||||||||||||||||||||||||||
| |||||||||||||||||||||||||||||||||||||