Yes, if you want to manually type in the normalization factors, you should create a report in the Document Grid that includes the "Explicit Global Standard Area" column.

You should fill in the Explicit Global Standard Area column with the values that you would like Skyline to divide by for normalization.

After you have filled in the Explicit Global Standard Area value for at least one file, you will be able to go to "Settings > Peptide Settings > Quantification" and choose "Ratio to Global Standards" as the "Normalization Method".
-- Nick

Thanks for the quick response! I noticed that once switched to ratio to global standards as normalization method, it wouldn't do normalization to heavy std. We have same amount of heavy standard spiked into the matrix. Would it be possible to keep normalization to heavy std and then normalize to that global factor?

Thanks,

I think the "Calculated Concentration" column will end up having the values that you want if you set the "Internal Standard Concentration" and "Sample Dilution Factor" columns.

The usual use case for "Sample Dilution Factor" is for when you know that your analyte's concentration is going to be above the linear range of the mass spectrometer and you dilute your sample by a known amount.
Then, you set the "Sample Dilution Factor" values so that the "Calculated Concentration" values that Skyline reports will be multiplied by the dilution factor.

Usually, the "Calculated Concentration" value is blank in Skyline unless you have a calibration curve, but, if you set the "Internal Standard Concentration" values to a number (e.g. "1") for each peptide, then the "Calculated Concentration" values will be equal to the "Normalized Area" values times the Internal Standard times the Replicate's Sample Dilution Factor.

-- Nick