Hello everyone,

First of all, I'd like to thank you for all your hard work. Skyline is a very powerful tool (even if I haven't mastered it perfectly yet) and it allows us to do so many things.

I have a challenge that I think is quite unique. I'm working in diaPASEF on a timsTOF Pro and I'm looking to do absolute quantization in label free mode.
I'd like to know if it's possible to make a calibration/spreading curve on diaPASEF acquisitions from a DIA-NN library?
If so, can you tell me how?

Thanks in advance!