Left-handed switching between ‘tabs’ for many measurements

support
Left-handed switching between ‘tabs’ for many measurements niklas gombert  2024-07-29 05:05
 

Dear Skyline team,

We really enjoy using your software to analyse our PRM measurements. However, there is one thing which caused us some trouble when dealing with larger data-sets. You are probably already familiar with the phenomenon - you create a method based on an initial measurement, validate it and start a big sample set.

However, in our case (and I can imagine that we won't be the only ones affected), we sometimes have slight shifts in our chromatograms when measuring several hundreds of samples. No problem - the PRM windows are large enough. Unfortunately, however, it can happen that the integration of the peaks does not always work automatically in these cases. Therefore, in the past we have often had to reintegrate ‘by hand’...it would therefore be an absolute quality of life improvement for us if we could ‘jump’ between the ‘tabs’ (i.e. between the individual measurements) in Skyline with our left hand, while we could integrate or reintegrate with the right hand (using the mouse). We know the "ALT + arrow key"-way, but this can only be done left-handed by real piano players ;)

Thank you very much in advance!

 
 
Nick Shulman responded:  2024-07-29 10:06
The easiest thing for you to do might be to use a program such as "AutoHotKey" to make it so that one of the Function keys on the keyboard behaves like "Alt + arrow key".
https://www.autohotkey.com/

I am supposed to make Skyline's peak picking better so that you do not have to do as much manual adjustment.
I made a poster for the ASMS conference in June showing how it might work (attached).
You can install a special build of Skyline with that functionality here:
https://proteome.gs.washington.edu/~nicksh/SpecialSkylines/PeakImputation/
This special builds of Skyline adds a "Edit > Integration > Peak Imputation" menu item.

If you could send us some of your datasets where you are having to do a lot of manual adjustment it might help us improve this feature.
In Skyline you can use the menu item:
File > Share
to create a .zip file containing your Skyline document and supporting files including extracted chromatograms.
Files which are less than 50MB can be attached to these support requests. You can always upload larger files here:
https://skyline.ms/files.url
-- Nick