Can I export heavy peptide areas in Report?

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Can I export heavy peptide areas in Report? martinnm  2024-07-27 18:27
 

Hi Skyline Team,

I want to export the normalized peak areas of heavy and light versions of my target peptides in my Report. I know I can customize my report and include "RatioLightToHeavy" but I was wondering if there is a selection for the normalized area specifically for the heavy peptide?

Thanks!

 
 
Nick Shulman responded:  2024-07-27 18:53
The column that you would want to include in your report is "Total Area".
"Total Area" is found on the Precursor Result, so you can find it in the Report Editor at:
Proteins > Peptides > Precursor > Precursor Results
(there's also a binoculars button which lets you search for any column by name).

When "Total Area" is included on your report you will get at least one row per Precursor, which typically means two rows per Peptide.
If you would like the heavy and light Total Area values to appear in the same row, you should check the checkbox "Pivot Isotope Label" in the Report Editor.

I am not sure what you mean by "normalized area for the heavy peptide".
We typically think that both the light area and the heavy area values are unnormalized. The normalized area is what you get when you divide the light area by the heavy area.
What sort of normalization were you thinking of?

The Custom Reports tutorial is also a great place to learn about reports:
https://skyline.ms/wiki/home/software/Skyline/page.view?name=tutorial_custom_reports
-- Nick
 
1748362947 responded:  2024-10-15 19:21