Hi team,

I had a few basic doubts regarding developing the PRM method as I am working on it for the first time. What are the points/parameters mandatory during a method development? (Ex: pooled QC, intraday, interday variations, calibration curve, reverse calibration curve). Should I run in triplicates when I have to check the sensitivity and specificity of the assay using serum samples?

Hoping for a positive response.

Thank you.
Akhila