• Sign In
  1. Requests

Trouble importing from Agilent ".D" files

support

View Request

view list print
Trouble importing from Agilent ".D" files dhass  2024-06-25 13:13
 
Hi,

I normally do GC-MS using an Agilent 5975 GC-MS system, and the data is in a ".D" format. The old Agilent data analysis software we have is terrible and I'd love to use Skyline, which I've been able to use to analyze my more occasional LC-MS experiments.

I know the ".D" format isn't one of the types imported into skyline, but I would really appreciate any advice I can get on making my files importable into skyline. In the past I've been able to use OpenChrom to import then export my files (then import them into Skyline), but they seem to somehow lose spectral information along the way.

Does anyone have any advice on a good way to go about converting my files?

Dan
 
 
Nick Shulman responded:  2024-06-25 13:46
Skyline does support some types of data in Agilent ".d" folders.

In order for a folder to be recognized as an Agilent folder, the folder name must end with ".d" and the folder must itself contain a folder named "AcqData", and that AcqData folder must contain either a file called "mspeak.bin" or "msprofile.bin".

If Skyline does not understand your data then I believe that ProteoWizard's "MSConvert" and "SeeMS.exe" would not understand it either.

Some people have successfully used Skyline with GC-MS data, but I do not know whether they did anything special to handle the fact that it was not LC-MS.
-- Nick