Skyline crashes when trying to add peptide modifications

Skyline crashes when trying to add peptide modifications areiter30500  2024-05-30 20:03

Hi, I'm unable to add any modifications to existing peptides in the target list. When I right-click the peptide and "Modify...", there is no change to the peptide or m/z values below. When I create a copy with a new modification, it just adds the exact same peptide below. Also when I right-click and select "Pick children", I get the "(Not responding)" error and the software crashes.

This used to work perfectly fine. I've been using Skyline-daily, and it was working fine last week. I updated to and it still doesn't work. I also uninstalled and reinstalled, but that didn't work either.

I've been using Skyline for a long time, and have never encountered any issues like this.

Thank you for your help!

Nick Shulman responded:  2024-05-30 20:36
Can you send us your Skyline document?
In Skyline you can use the menu item:
File > Share
to create a .zip file containing your Skyline document and supporting files including extracted chromatograms and spectral libraries.

If that .zip file is less than 50MB you can attach it to this support request. You can upload larger files here:

What exactly do you mean when you say it "crashes"? Are you saying that Skyline disappears?
Sometimes, after Skyline abruptly exits, the next time that you start up Skyline it tells you that there was an error and gives you the opportunity to send an error report to us. It is always very helpful when you send us those error reports.

If you don't get a message like that the next time you start Skyline then sometimes there is useful information in the Windows Event Viewer.

But, hopefully, once I see your Skyline document I will have some better theories about what might be going wrong.
-- Nick