disulfide crosslinked homodimeric also covalently modified peptide does not appear to work | warham | 2024-05-14 08:08 | |||||||||||||||||||||||||||||||||||||||||
So this will probably go to Nick, So disulfide homodimeric peptides load beautifully through the modify peptide interface. And crosslinks between different peptides can also take other chemical modifications in the insert peptide interface. On a mAb there is this sequence: THTCPPCPAPELLGGPSVFLFPPKPK And in evaluating it's disulfide integrity, this peptide THTCPPC[+57]PAPELLGGPSVFLFPPKPK-THTCPPC[+57]PAPELLGGPSVFLFPPKPK-[DSS@4,4] would be useful but I get the error that the peptide is not recognized by the software. I tried adding another copy of the sequence but there is no field for specifying different proteins in the same peptide entry on the insert peptide field. Also the insert peptide field does not seem to support multiple crosslinks between two peptides which does happen with disulfides. Is there a way to do this that I'm missing? If it's too specific an issue, that's fine. But I thought that since there are a fair number of disulfide oligomers, it might affect others too. Best, |
|||||||||||||||||||||||||||||||||||||||||||
| |||||||||||||||||||||||||||||||||||||||||||