Quantification with a surrogate analyte

Quantification with a surrogate analyte dsgk  2024-04-24 03:55

Hi Skyline Team,

I have a biomarker assay where I'm using two differently labelled heavy peptides - one is used as an internal standard, the other as the surrogate analyte used for calibration (because my blank matrix has the light peptide endogenously present). I can't figure out how to get Skyline use the calibration curve of the surrogate analyte for calculating the concentrations of my unknown samples where I have signals coming only from the light peptide and the internal standard. Any ideas how to get Skyline to do this?


Nick Shulman responded:  2024-04-24 10:30
A new feature was added to Skyline-daily a few months ago which allows you to specify that a molecule should use the calibration curve from a different molecule.
The column that you would need to show in the Document Grid is "Surrogate External Standard".
There is a little bit of information about how to use this feature here:
-- Nick