Hi Skyline Team,

I have a biomarker assay where I'm using two differently labelled heavy peptides - one is used as an internal standard, the other as the surrogate analyte used for calibration (because my blank matrix has the light peptide endogenously present). I can't figure out how to get Skyline use the calibration curve of the surrogate analyte for calculating the concentrations of my unknown samples where I have signals coming only from the light peptide and the internal standard. Any ideas how to get Skyline to do this?

Thanks!