I am trying to quantify a protein and I have loaded its sequence in skyline to in silico digest it and filter peptides. I have then created a library using prosit and loaded the raw data and picked the peptides that have good signal, peak shape, good dotp with the prosit library (I ignored the RT component of prosit prediction).

Now that I have a list of high-quality peptides from real data, I would like to use the data to create a new library using the experimentally observed RT and fragmentation spectra. However, I can't find a way to do this.

Hence, my question is: how do I take the experimentally observed data loaded into the skyline and curated there to create a library?