Importing fractionation sample to Skyline

Importing fractionation sample to Skyline GH  2024-02-15 13:23

I have DIA sample which is fractionated and I have total of 12 .raw fractions data files.
My question is how can import raw files to Skyline? is there anyway to import as combine all fractions? or we need to combine before importing?

Thank you, Ghazaleh

Nick Shulman responded:  2024-02-15 13:47
When you do "File > Import > Results" the first window that comes up gives you options about how to combine multiple files into a single replicate. It sounds like you should choose the "Add one new replicate" option and then select your twelve raw files in the next window.