Hi Skyline Team,
I have been trying to develop a PRM method for a targeted DIA experiment in skyline. I have eight targets of interest which have been built into a transition list, but I do not expect all my samples to have all 8 of these targets. I have been successful in building the calibration curves from spectra for my targets and setting up the iRT calculator, however, when attempting to upload results for sample quantification, I keep receiving this error:
"Failed importing results file.
The calculator requires all 8 of its standard peptides to be in the targets list in order to determine a regression."
I have followed the PRM and iRT tutorials and looked through other support requests, but unfortunately have not been able to fix this. The tutorial data works without any trouble, but once applied to my own spectra throws an error. I have attempted to re-import spectra, and rebuild the calculators, but the same error still occurs. Any advice/help would be greatly appreciated!
Best,
Lauren