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How to get to the EIC plot that was shown when importing the result?

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How to get to the EIC plot that was shown when importing the result? Lavender  2023-11-14 16:36
 

Hi,

I am sure this is a very basic question, but I have spent half of the day searching and couldn't find the answer, so thank you ahead for being patient with me.

I am given a skyline template for 20 peptides. I found the EIC plot (attached) that was shown when importing results were in progress very useful to have an overall idea of the runs, especially when each peak has a different color.

These are my questions:

  1. I assume this is an EIC plot, that extracted the m/z of the 20 targets in the skyline template. Is that correct?
  2. Is there a way I can revisit the plot and export the information, such as the area/signal intensity, RT, and correlated m/z or peptides sequence of a peak?

Thank you so much!

How do I revisit the EIC plot?
And is the EIC plot of the targets m/z in the files?
 
 
Nick Shulman responded:  2023-11-14 16:46
If you would like to learn how to export lists of numbers from Skyline, you should look at the Custom Reports tutorial:
https://skyline.ms/wiki/home/software/Skyline/page.view?name=tutorial_custom_reports

Skyline will only show you the graph in your screenshot while Skyline is in the process of extracting chromatograms.

You can see a graph which looks similar to that if you select more than one Peptide in the Targets tree.
If you have selected between two and one hundred peptides in the Targets tree, then the Chromatogram graph will display a single chromatogram for each of the selected peptides.
If you have more than 100 peptides then the Chromatogram graph will be blank because that ends up being too much data for Skyline to load at once.

-- Nick