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Chromatogram was unable haihaba  2023-10-29 10:46
 

Hello,

My data was generated from Thermo Quantis Plus and I used Skyline 23.1.

Some transition chromatograms were not visible. One interesting thing to note is that several compounds have the same chemical formula, but while some can be seen in the chromatograms, others do not appear to have the same Q1 and Q3 transitions.

Please see the attached files.

Thanks
 
 
Nick Shulman responded:  2023-10-29 10:58
The problem is probably that the "Explicit Retention Time" value that has been specified for the molecule does not actually overlap with the retention time range over which the SRM chromatogram was acquired.
If the Explicit Retention Time does not overlap with the SRM chromatogram, then Skyline assumes that the SRM chromatogram was not intended to be used by that particular molecule, and so you get the "Chromatogram information unavailable method".

You can use the "Molecules" report in the Document Grid to change the Explicit Retention Time values. You can either set the Explicit Retention Time values for these molecules to be blank, or change the number so that it is within the retention time range of the acquired chromatogram.
-- Nick
 
haihaba responded:  2023-10-30 17:10
Thank you, Nick. It works well.