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ERROR: Generating Skyline library using Peaks outputs (bb.pep.xml or db.mzidentml.xml)

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ERROR: Generating Skyline library using Peaks outputs (bb.pep.xml or db.mzidentml.xml) jthalles  2023-10-26 19:18
 

Dear Skyliner's,

I'm trying to build a spectral library using PEAKS v.11 outputs (db.pep.xml or db.mzidentml.xml).

The data was acquired using ETHCD (b, y, c, z, z') to map 3 peptides with isotopic label

After importing the pep.xml file in peptide setting > library > build, I receive an error message. Information attached

Coudl you help me, please?

Best
Thalles
 
 
Nick Shulman responded:  2023-10-26 19:39
That error message is saying that it was trying to find a file named "231025_AC11_AA011RaspN_heavyNPNT_tMS2_autoAGCandMaxIT.raw".

Do you have a file named "231025_AC11_AA011RaspN_heavyNPNT_tMS2_autoAGCandMaxIT.raw"?
If so, you should copy that file into the same folder as the file "db.pep.xml".

The error message that you got can sometimes be confusing.
The error message is trying to tell you that it was looking for a file whose name ends with any of a long list of possible filename extensions including ".mz5", ".mzML", ".raw" and whose name started with "231025_AC11_AA011RaspN_heavyNPNT_tMS2_autoAGCandMaxIT".

The error message is also letting you know that it tried looking in a number of different folders, but if you put that .raw file in the same folder as your db.pep.xml file, it would probably be simplest.
-- Nick
 
jthalles responded:  2023-10-27 10:34
HI Nick,

Gotcha. It's works now.

Thanks a lot.

Thalles