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an error of prm-pasef method about retention time

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an error of prm-pasef method about retention time 931080630  2023-10-18 20:26
 
Dear support team,

     There is a problem when i edited a prm-pasef method. Obviously, i have already edited the retention time of each peptide , but when i export the method to the tims-control software, the retention time of every peptide are the same. Not only for me, but also some other people accounted the same issue(see the attached picture） i am looking forward to receive your reply.

   Best regards!
   Yang
 
 
Nick Shulman responded:  2023-10-18 21:02
Can you send us your Skyline document?

In Skyline you can use the menu item:
File > Share
to create a .zip file containing your Skyline document and supporting files including spectral and ion mobility libraries.

If that .zip file is less than 50MB you can attach it to this support request. You can upload larger files here:
https://skyline.ms/files.url

It would also be helpful if you could show us a screenshot of the Export Method dialog so that we can see what settings you are specifying, and also please send us the method template file that you are using.
--Nick
 
931080630 responded:  2023-10-18 21:52
Thanks for your timely reply. The .zip file is attached, if you need other documents, please inform me anytime.

   Best regards!
   Yang