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Export Method>SciexOS 7500 Error

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Export Method>SciexOS 7500 Error Dhaval Patel  2023-10-13 09:48
 
Hello,
We are getting a frequent error exporting skyline MRM transition to SciexOS 7500 method. Please find the following error message. I have also attached the skyline file and Sciex)S 7500 template for your quick reference.

Please do not hesitate to ask me if you have any questions or need any further information.

Thank you
Dhaval
Texas Tech University

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Skyline
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An error occurred attempting to export.
ERROR: Failure creating method file D:\SCIEX OS Data\Dr German_ Inflammatory Proteins\Acquisition Methods\TNF_Skyline MRM 135.msm. MsMethodValidationResponse failure.
Error code: DeviceMethods-MethodsIsNotValidDueToValidationErrors.
Error message: Context=Skyline Template-MRM-TableWithRowCount=135-RowIndex-18, Property=CompoundIdProperty, Value = Tumor necrosis factor.TPSDKPVAHVVANPQAEGQLQWLNR.+3y8.light; Context=Skyline Template-MRM-TableWithRowCount=135-RowIndex-19, Property=CompoundIdProperty, Value = Tumor necrosis factor.TPSDKPVAHVVANPQAEGQLQWLNR.+3y7.light; Context=Skyline Template-MRM-TableWithRowCount=135-RowIndex-20, Property=CompoundIdProperty, Value = Tumor necrosis factor.TPSDKPVAHVVANPQAEGQLQWLNR.+3y6.light; Context=Skyline Template-MRM-TableWithRowCount=135-RowIndex-21, Property=CompoundIdProperty, Value = Tumor necrosis factor.TPSDKPVAHVVANPQAEGQLQWLNR.+3y5.light; Context=Skyline Template-MRM-TableWithRowCount=135-RowIndex-22, Property=CompoundIdProperty, Value = Tumor necrosis factor.TPSDKPVAHVVANPQAEGQLQWLNR.+3y4.light; Context=Skyline Template-MRM-TableWithRowCount=135-RowIndex-23, Property=CompoundIdProperty, Value = Tumor necrosis factor.TPSDKPVAHVVANPQAEGQLQWLNR.+3y3.light; Context=Skyline Template-MRM-TableWithRowCount=135-RowIndex-24, Property=CompoundIdProperty, Value = Tumor necrosis factor.TPSDKPVAHVVANPQAEGQLQWLNR.+3y2.light; Context=Skyline Template-MRM-TableWithRowCount=135-RowIndex-25, Property=CompoundIdProperty, Value = Tumor necrosis factor.TPSDKPVAHVVANPQAEGQLQWLNR.+3b2.light; Context=Skyline Template-MRM-TableWithRowCount=135-RowIndex-26, Property=CompoundIdProperty, Value = Tumor necrosis factor.TPSDKPVAHVVANPQAEGQLQWLNR.+3b3.light; Context=Skyline Template-MRM-TableWithRowCount=135-RowIndex-27, Property=CompoundIdProperty, Value = Tumor necrosis factor.TPSDKPVAHVVANPQAEGQLQWLNR.+3b4.light; Context=Skyline Template-MRM-TableWithRowCount=135-RowIndex-28, Property=CompoundIdProperty, Value = Tumor necrosis factor.TPSDKPVAHVVANPQAEGQLQWLNR.+3b5.light; Context=Skyline Template-MRM-TableWithRowCount=135-RowIndex-29, Property=CompoundIdProperty, Value = Tumor necrosis factor.TPSDKPVAHVVANPQAEGQLQWLNR.+3b6.light; Context=Skyline Template-MRM-TableWithRowCount=135-RowIndex-30, Property=CompoundIdProperty, Value = Tumor necrosis factor.TPSDKPVAHVVANPQAEGQLQWLNR.+3b7.light; Context=Skyline Template-MRM-TableWithRowCount=135-RowIndex-31, Property=CompoundIdProperty, Value = Tumor necrosis factor.TPSDKPVAHVVANPQAEGQLQWLNR.+3b8.light; Context=Skyline Template-MRM-TableWithRowCount=135-RowIndex-32, Property=CompoundIdProperty, Value = Tumor necrosis factor.TPSDKPVAHVVANPQAEGQLQWLNR.+3b9.light; Context=Skyline Template-MRM-TableWithRowCount=135-RowIndex-33, Property=CompoundIdProperty, Value = Tumor necrosis factor.TPSDKPVAHVVANPQAEGQLQWLNR.+3b10.light; Context=Skyline Template-MRM-TableWithRowCount=135-RowIndex-50, Property=CompoundIdProperty, Value = Tumor necrosis factor.DNQLVVPSEGLYLIYSQVLFK.+3y8.light; Context=Skyline Template-MRM-TableWithRowCount=135-RowIndex-51, Property=CompoundIdProperty, Value = Tumor necrosis factor.DNQLVVPSEGLYLIYSQVLFK.+3y7.light; Context=Skyline Template-MRM-TableWithRowCount=135-RowIndex-52, Property=CompoundIdProperty, Value = Tumor necrosis factor.DNQLVVPSEGLYLIYSQVLFK.+3y6.light; Context=Skyline Template-MRM-TableWithRowCount=135-RowIndex-53, Property=CompoundIdProperty, Value = Tumor necrosis factor.DNQLVVPSEGLYLIYSQVLFK.+3y5.light; Context=Skyline Template-MRM-TableWithRowCount=135-RowIndex-54, Property=CompoundIdProperty, Value = Tumor necrosis factor.DNQLVVPSEGLYLIYSQVLFK.+3y4.light; Context=Skyline Template-MRM-TableWithRowCount=135-RowIndex-55, Property=CompoundIdProperty, Value = Tumor necrosis factor.DNQLVVPSEGLYLIYSQVLFK.+3y3.light; Context=Skyline Template-MRM-TableWithRowCount=135-RowIndex-56, Property=CompoundIdProperty, Value = Tumor necrosis factor.DNQLVVPSEGLYLIYSQVLFK.+3y2.light; Context=Skyline Template-MRM-TableWithRowCount=135-RowIndex-57, Property=CompoundIdProperty, Value = Tumor necrosis factor.DNQLVVPSEGLYLIYSQVLFK.+3b2.light; Context=Skyline Template-MRM-TableWithRowCount=135-RowIndex-58, Property=CompoundIdProperty, Value = Tumor necrosis factor.DNQLVVPSEGLYLIYSQVLFK.+3b3.light; Context=Skyline Template-MRM-TableWithRowCount=135-RowIndex-59, Property=CompoundIdProperty, Value = Tumor necrosis factor.DNQLVVPSEGLYLIYSQVLFK.+3b4.light; Context=Skyline Template-MRM-TableWithRowCount=135-RowIndex-60, Property=CompoundIdProperty, Value = Tumor necrosis factor.DNQLVVPSEGLYLIYSQVLFK.+3b5.light; Context=Skyline Template-MRM-TableWithRowCount=135-RowIndex-61, Property=CompoundIdProperty, Value = Tumor necrosis factor.DNQLVVPSEGLYLIYSQVLFK.+3b6.light; Context=Skyline Template-MRM-TableWithRowCount=135-RowIndex-62, Property=CompoundIdProperty, Value = Tumor necrosis factor.DNQLVVPSEGLYLIYSQVLFK.+3b7.light; Context=Skyline Template-MRM-TableWithRowCount=135-RowIndex-63, Property=CompoundIdProperty, Value = Tumor necrosis factor.DNQLVVPSEGLYLIYSQVLFK.+3b8.light; Context=Skyline Template-MRM-TableWithRowCount=135-RowIndex-64, Property=CompoundIdProperty, Value = Tumor necrosis factor.DNQLVVPSEGLYLIYSQVLFK.+3b9.light; Context=Skyline Template-MRM-TableWithRowCount=135-RowIndex-65, Property=CompoundIdProperty, Value = Tumor necrosis factor.DNQLVVPSEGLYLIYSQVLFK.+3b10.light; Context=Skyline Template-MRM-TableWithRowCount=135-RowIndex-82, Property=CompoundIdProperty, Value = Tumor necrosis factor.GQGCPSTHVLLTHTISR.+2b10.light; Context=Skyline Template-MRM-TableWithRowCount=135-RowIndex-83, Property=CompoundIdProperty, Value = Tumor necrosis factor.GQGCPSTHVLLTHTISR.+2b11.light; Context=Skyline Template-MRM-TableWithRowCount=135-RowIndex-100, Property=CompoundIdProperty, Value = Tumor necrosis factor.GQGCPSTHVLLTHTISR.+3b10.light; Context=Skyline Template-MRM-TableWithRowCount=135-RowIndex-101, Property=CompoundIdProperty, Value = Tumor necrosis factor.GQGCPSTHVLLTHTISR.+3b11.light; Context=Skyline Template-MRM-TableWithRowCount=135-RowIndex-118, Property=CompoundIdProperty, Value = Tumor necrosis factor.ETPEGAEAKPWYEPIYLGGVFQLEK.+3y9.light; Context=Skyline Template-MRM-TableWithRowCount=135-RowIndex-119, Property=CompoundIdProperty, Value = Tumor necrosis factor.ETPEGAEAKPWYEPIYLGGVFQLEK.+3y8.light; Context=Skyline Template-MRM-TableWithRowCount=135-RowIndex-120, Property=CompoundIdProperty, Value = Tumor necrosis factor.ETPEGAEAKPWYEPIYLGGVFQLEK.+3y7.light; Context=Skyline Template-MRM-TableWithRowCount=135-RowIndex-121, Property=CompoundIdProperty, Value = Tumor necrosis factor.ETPEGAEAKPWYEPIYLGGVFQLEK.+3y6.light; Context=Skyline Template-MRM-TableWithRowCount=135-RowIndex-122, Property=CompoundIdProperty, Value = Tumor necrosis factor.ETPEGAEAKPWYEPIYLGGVFQLEK.+3y5.light; Context=Skyline Template-MRM-TableWithRowCount=135-RowIndex-123, Property=CompoundIdProperty, Value = Tumor necrosis factor.ETPEGAEAKPWYEPIYLGGVFQLEK.+3y4.light; Context=Skyline Template-MRM-TableWithRowCount=135-RowIndex-124, Property=CompoundIdProperty, Value = Tumor necrosis factor.ETPEGAEAKPWYEPIYLGGVFQLEK.+3y3.light; Context=Skyline Template-MRM-TableWithRowCount=135-RowIndex-125, Property=CompoundIdProperty, Value = Tumor necrosis factor.ETPEGAEAKPWYEPIYLGGVFQLEK.+3y2.light; Context=Skyline Template-MRM-TableWithRowCount=135-RowIndex-126, Property=CompoundIdProperty, Value = Tumor necrosis factor.ETPEGAEAKPWYEPIYLGGVFQLEK.+3b2.light; Context=Skyline Template-MRM-TableWithRowCount=135-RowIndex-127, Property=CompoundIdProperty, Value = Tumor necrosis factor.ETPEGAEAKPWYEPIYLGGVFQLEK.+3b3.light; Context=Skyline Template-MRM-TableWithRowCount=135-RowIndex-128, Property=CompoundIdProperty, Value = Tumor necrosis factor.ETPEGAEAKPWYEPIYLGGVFQLEK.+3b4.light; Context=Skyline Template-MRM-TableWithRowCount=135-RowIndex-129, Property=CompoundIdProperty, Value = Tumor necrosis factor.ETPEGAEAKPWYEPIYLGGVFQLEK.+3b5.light; Context=Skyline Template-MRM-TableWithRowCount=135-RowIndex-130, Property=CompoundIdProperty, Value = Tumor necrosis factor.ETPEGAEAKPWYEPIYLGGVFQLEK.+3b6.light; Context=Skyline Template-MRM-TableWithRowCount=135-RowIndex-131, Property=CompoundIdProperty, Value = Tumor necrosis factor.ETPEGAEAKPWYEPIYLGGVFQLEK.+3b7.light; Context=Skyline Template-MRM-TableWithRowCount=135-RowIndex-132, Property=CompoundIdProperty, Value = Tumor necrosis factor.ETPEGAEAKPWYEPIYLGGVFQLEK.+3b8.light; Context=Skyline Template-MRM-TableWithRowCount=135-RowIndex-133, Property=CompoundIdProperty, Value = Tumor necrosis factor.ETPEGAEAKPWYEPIYLGGVFQLEK.+3b9.light; Context=Skyline Template-MRM-TableWithRowCount=135-RowIndex-134, Property=CompoundIdProperty, Value = Tumor necrosis factor.ETPEGAEAKPWYEPIYLGGVFQLEK.+3b10.light.

Command-line: Method\AbSciex\SciexOS\BuildSciexMethod -d -m "D:\SCIEX OS Data\Dhaval_Proteins\Acquisition Methods\Skyline Template.msm" "D:\SCIEX OS Data\Dr German_ Inflammatory Proteins\Acquisition Methods\qx5pnf2o.wbd\transitions.txt"
Working directory: D:\SCIEX OS Data\Dr German_ Inflammatory Proteins\Acquisition Methods
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OK More Info
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System.IO.IOException: ERROR: Failure creating method file D:\SCIEX OS Data\Dr German_ Inflammatory Proteins\Acquisition Methods\TNF_Skyline MRM 135.msm. MsMethodValidationResponse failure.
Error code: DeviceMethods-MethodsIsNotValidDueToValidationErrors.
Error message: Context=Skyline Template-MRM-TableWithRowCount=135-RowIndex-18, Property=CompoundIdProperty, Value = Tumor necrosis factor.TPSDKPVAHVVANPQAEGQLQWLNR.+3y8.light; Context=Skyline Template-MRM-TableWithRowCount=135-RowIndex-19, Property=CompoundIdProperty, Value = Tumor necrosis factor.TPSDKPVAHVVANPQAEGQLQWLNR.+3y7.light; Context=Skyline Template-MRM-TableWithRowCount=135-RowIndex-20, Property=CompoundIdProperty, Value = Tumor necrosis factor.TPSDKPVAHVVANPQAEGQLQWLNR.+3y6.light; Context=Skyline Template-MRM-TableWithRowCount=135-RowIndex-21, Property=CompoundIdProperty, Value = Tumor necrosis factor.TPSDKPVAHVVANPQAEGQLQWLNR.+3y5.light; Context=Skyline Template-MRM-TableWithRowCount=135-RowIndex-22, Property=CompoundIdProperty, Value = Tumor necrosis factor.TPSDKPVAHVVANPQAEGQLQWLNR.+3y4.light; Context=Skyline Template-MRM-TableWithRowCount=135-RowIndex-23, Property=CompoundIdProperty, Value = Tumor necrosis factor.TPSDKPVAHVVANPQAEGQLQWLNR.+3y3.light; Context=Skyline Template-MRM-TableWithRowCount=135-RowIndex-24, Property=CompoundIdProperty, Value = Tumor necrosis factor.TPSDKPVAHVVANPQAEGQLQWLNR.+3y2.light; Context=Skyline Template-MRM-TableWithRowCount=135-RowIndex-25, Property=CompoundIdProperty, Value = Tumor necrosis factor.TPSDKPVAHVVANPQAEGQLQWLNR.+3b2.light; Context=Skyline Template-MRM-TableWithRowCount=135-RowIndex-26, Property=CompoundIdProperty, Value = Tumor necrosis factor.TPSDKPVAHVVANPQAEGQLQWLNR.+3b3.light; Context=Skyline Template-MRM-TableWithRowCount=135-RowIndex-27, Property=CompoundIdProperty, Value = Tumor necrosis factor.TPSDKPVAHVVANPQAEGQLQWLNR.+3b4.light; Context=Skyline Template-MRM-TableWithRowCount=135-RowIndex-28, Property=CompoundIdProperty, Value = Tumor necrosis factor.TPSDKPVAHVVANPQAEGQLQWLNR.+3b5.light; Context=Skyline Template-MRM-TableWithRowCount=135-RowIndex-29, Property=CompoundIdProperty, Value = Tumor necrosis factor.TPSDKPVAHVVANPQAEGQLQWLNR.+3b6.light; Context=Skyline Template-MRM-TableWithRowCount=135-RowIndex-30, Property=CompoundIdProperty, Value = Tumor necrosis factor.TPSDKPVAHVVANPQAEGQLQWLNR.+3b7.light; Context=Skyline Template-MRM-TableWithRowCount=135-RowIndex-31, Property=CompoundIdProperty, Value = Tumor necrosis factor.TPSDKPVAHVVANPQAEGQLQWLNR.+3b8.light; Context=Skyline Template-MRM-TableWithRowCount=135-RowIndex-32, Property=CompoundIdProperty, Value = Tumor necrosis factor.TPSDKPVAHVVANPQAEGQLQWLNR.+3b9.light; Context=Skyline Template-MRM-TableWithRowCount=135-RowIndex-33, Property=CompoundIdProperty, Value = Tumor necrosis factor.TPSDKPVAHVVANPQAEGQLQWLNR.+3b10.light; Context=Skyline Template-MRM-TableWithRowCount=135-RowIndex-50, Property=CompoundIdProperty, Value = Tumor necrosis factor.DNQLVVPSEGLYLIYSQVLFK.+3y8.light; Context=Skyline Template-MRM-TableWithRowCount=135-RowIndex-51, Property=CompoundIdProperty, Value = Tumor necrosis factor.DNQLVVPSEGLYLIYSQVLFK.+3y7.light; Context=Skyline Template-MRM-TableWithRowCount=135-RowIndex-52, Property=CompoundIdProperty, Value = Tumor necrosis factor.DNQLVVPSEGLYLIYSQVLFK.+3y6.light; Context=Skyline Template-MRM-TableWithRowCount=135-RowIndex-53, Property=CompoundIdProperty, Value = Tumor necrosis factor.DNQLVVPSEGLYLIYSQVLFK.+3y5.light; Context=Skyline Template-MRM-TableWithRowCount=135-RowIndex-54, Property=CompoundIdProperty, Value = Tumor necrosis factor.DNQLVVPSEGLYLIYSQVLFK.+3y4.light; Context=Skyline Template-MRM-TableWithRowCount=135-RowIndex-55, Property=CompoundIdProperty, Value = Tumor necrosis factor.DNQLVVPSEGLYLIYSQVLFK.+3y3.light; Context=Skyline Template-MRM-TableWithRowCount=135-RowIndex-56, Property=CompoundIdProperty, Value = Tumor necrosis factor.DNQLVVPSEGLYLIYSQVLFK.+3y2.light; Context=Skyline Template-MRM-TableWithRowCount=135-RowIndex-57, Property=CompoundIdProperty, Value = Tumor necrosis factor.DNQLVVPSEGLYLIYSQVLFK.+3b2.light; Context=Skyline Template-MRM-TableWithRowCount=135-RowIndex-58, Property=CompoundIdProperty, Value = Tumor necrosis factor.DNQLVVPSEGLYLIYSQVLFK.+3b3.light; Context=Skyline Template-MRM-TableWithRowCount=135-RowIndex-59, Property=CompoundIdProperty, Value = Tumor necrosis factor.DNQLVVPSEGLYLIYSQVLFK.+3b4.light; Context=Skyline Template-MRM-TableWithRowCount=135-RowIndex-60, Property=CompoundIdProperty, Value = Tumor necrosis factor.DNQLVVPSEGLYLIYSQVLFK.+3b5.light; Context=Skyline Template-MRM-TableWithRowCount=135-RowIndex-61, Property=CompoundIdProperty, Value = Tumor necrosis factor.DNQLVVPSEGLYLIYSQVLFK.+3b6.light; Context=Skyline Template-MRM-TableWithRowCount=135-RowIndex-62, Property=CompoundIdProperty, Value = Tumor necrosis factor.DNQLVVPSEGLYLIYSQVLFK.+3b7.light; Context=Skyline Template-MRM-TableWithRowCount=135-RowIndex-63, Property=CompoundIdProperty, Value = Tumor necrosis factor.DNQLVVPSEGLYLIYSQVLFK.+3b8.light; Context=Skyline Template-MRM-TableWithRowCount=135-RowIndex-64, Property=CompoundIdProperty, Value = Tumor necrosis factor.DNQLVVPSEGLYLIYSQVLFK.+3b9.light; Context=Skyline Template-MRM-TableWithRowCount=135-RowIndex-65, Property=CompoundIdProperty, Value = Tumor necrosis factor.DNQLVVPSEGLYLIYSQVLFK.+3b10.light; Context=Skyline Template-MRM-TableWithRowCount=135-RowIndex-82, Property=CompoundIdProperty, Value = Tumor necrosis factor.GQGCPSTHVLLTHTISR.+2b10.light; Context=Skyline Template-MRM-TableWithRowCount=135-RowIndex-83, Property=CompoundIdProperty, Value = Tumor necrosis factor.GQGCPSTHVLLTHTISR.+2b11.light; Context=Skyline Template-MRM-TableWithRowCount=135-RowIndex-100, Property=CompoundIdProperty, Value = Tumor necrosis factor.GQGCPSTHVLLTHTISR.+3b10.light; Context=Skyline Template-MRM-TableWithRowCount=135-RowIndex-101, Property=CompoundIdProperty, Value = Tumor necrosis factor.GQGCPSTHVLLTHTISR.+3b11.light; Context=Skyline Template-MRM-TableWithRowCount=135-RowIndex-118, Property=CompoundIdProperty, Value = Tumor necrosis factor.ETPEGAEAKPWYEPIYLGGVFQLEK.+3y9.light; Context=Skyline Template-MRM-TableWithRowCount=135-RowIndex-119, Property=CompoundIdProperty, Value = Tumor necrosis factor.ETPEGAEAKPWYEPIYLGGVFQLEK.+3y8.light; Context=Skyline Template-MRM-TableWithRowCount=135-RowIndex-120, Property=CompoundIdProperty, Value = Tumor necrosis factor.ETPEGAEAKPWYEPIYLGGVFQLEK.+3y7.light; Context=Skyline Template-MRM-TableWithRowCount=135-RowIndex-121, Property=CompoundIdProperty, Value = Tumor necrosis factor.ETPEGAEAKPWYEPIYLGGVFQLEK.+3y6.light; Context=Skyline Template-MRM-TableWithRowCount=135-RowIndex-122, Property=CompoundIdProperty, Value = Tumor necrosis factor.ETPEGAEAKPWYEPIYLGGVFQLEK.+3y5.light; Context=Skyline Template-MRM-TableWithRowCount=135-RowIndex-123, Property=CompoundIdProperty, Value = Tumor necrosis factor.ETPEGAEAKPWYEPIYLGGVFQLEK.+3y4.light; Context=Skyline Template-MRM-TableWithRowCount=135-RowIndex-124, Property=CompoundIdProperty, Value = Tumor necrosis factor.ETPEGAEAKPWYEPIYLGGVFQLEK.+3y3.light; Context=Skyline Template-MRM-TableWithRowCount=135-RowIndex-125, Property=CompoundIdProperty, Value = Tumor necrosis factor.ETPEGAEAKPWYEPIYLGGVFQLEK.+3y2.light; Context=Skyline Template-MRM-TableWithRowCount=135-RowIndex-126, Property=CompoundIdProperty, Value = Tumor necrosis factor.ETPEGAEAKPWYEPIYLGGVFQLEK.+3b2.light; Context=Skyline Template-MRM-TableWithRowCount=135-RowIndex-127, Property=CompoundIdProperty, Value = Tumor necrosis factor.ETPEGAEAKPWYEPIYLGGVFQLEK.+3b3.light; Context=Skyline Template-MRM-TableWithRowCount=135-RowIndex-128, Property=CompoundIdProperty, Value = Tumor necrosis factor.ETPEGAEAKPWYEPIYLGGVFQLEK.+3b4.light; Context=Skyline Template-MRM-TableWithRowCount=135-RowIndex-129, Property=CompoundIdProperty, Value = Tumor necrosis factor.ETPEGAEAKPWYEPIYLGGVFQLEK.+3b5.light; Context=Skyline Template-MRM-TableWithRowCount=135-RowIndex-130, Property=CompoundIdProperty, Value = Tumor necrosis factor.ETPEGAEAKPWYEPIYLGGVFQLEK.+3b6.light; Context=Skyline Template-MRM-TableWithRowCount=135-RowIndex-131, Property=CompoundIdProperty, Value = Tumor necrosis factor.ETPEGAEAKPWYEPIYLGGVFQLEK.+3b7.light; Context=Skyline Template-MRM-TableWithRowCount=135-RowIndex-132, Property=CompoundIdProperty, Value = Tumor necrosis factor.ETPEGAEAKPWYEPIYLGGVFQLEK.+3b8.light; Context=Skyline Template-MRM-TableWithRowCount=135-RowIndex-133, Property=CompoundIdProperty, Value = Tumor necrosis factor.ETPEGAEAKPWYEPIYLGGVFQLEK.+3b9.light; Context=Skyline Template-MRM-TableWithRowCount=135-RowIndex-134, Property=CompoundIdProperty, Value = Tumor necrosis factor.ETPEGAEAKPWYEPIYLGGVFQLEK.+3b10.light.

Command-line: Method\AbSciex\SciexOS\BuildSciexMethod -d -m "D:\SCIEX OS Data\Dhaval_Proteins\Acquisition Methods\Skyline Template.msm" "D:\SCIEX OS Data\Dr German_ Inflammatory Proteins\Acquisition Methods\qx5pnf2o.wbd\transitions.txt"
Working directory: D:\SCIEX OS Data\Dr German_ Inflammatory Proteins\Acquisition Methods ---> System.IO.IOException: ERROR: Failure creating method file D:\SCIEX OS Data\Dr German_ Inflammatory Proteins\Acquisition Methods\TNF_Skyline MRM 135.msm. MsMethodValidationResponse failure.
Error code: DeviceMethods-MethodsIsNotValidDueToValidationErrors.
Error message: Context=Skyline Template-MRM-TableWithRowCount=135-RowIndex-18, Property=CompoundIdProperty, Value = Tumor necrosis factor.TPSDKPVAHVVANPQAEGQLQWLNR.+3y8.light; Context=Skyline Template-MRM-TableWithRowCount=135-RowIndex-19, Property=CompoundIdProperty, Value = Tumor necrosis factor.TPSDKPVAHVVANPQAEGQLQWLNR.+3y7.light; Context=Skyline Template-MRM-TableWithRowCount=135-RowIndex-20, Property=CompoundIdProperty, Value = Tumor necrosis factor.TPSDKPVAHVVANPQAEGQLQWLNR.+3y6.light; Context=Skyline Template-MRM-TableWithRowCount=135-RowIndex-21, Property=CompoundIdProperty, Value = Tumor necrosis factor.TPSDKPVAHVVANPQAEGQLQWLNR.+3y5.light; Context=Skyline Template-MRM-TableWithRowCount=135-RowIndex-22, Property=CompoundIdProperty, Value = Tumor necrosis factor.TPSDKPVAHVVANPQAEGQLQWLNR.+3y4.light; Context=Skyline Template-MRM-TableWithRowCount=135-RowIndex-23, Property=CompoundIdProperty, Value = Tumor necrosis factor.TPSDKPVAHVVANPQAEGQLQWLNR.+3y3.light; Context=Skyline Template-MRM-TableWithRowCount=135-RowIndex-24, Property=CompoundIdProperty, Value = Tumor necrosis factor.TPSDKPVAHVVANPQAEGQLQWLNR.+3y2.light; Context=Skyline Template-MRM-TableWithRowCount=135-RowIndex-25, Property=CompoundIdProperty, Value = Tumor necrosis factor.TPSDKPVAHVVANPQAEGQLQWLNR.+3b2.light; Context=Skyline Template-MRM-TableWithRowCount=135-RowIndex-26, Property=CompoundIdProperty, Value = Tumor necrosis factor.TPSDKPVAHVVANPQAEGQLQWLNR.+3b3.light; Context=Skyline Template-MRM-TableWithRowCount=135-RowIndex-27, Property=CompoundIdProperty, Value = Tumor necrosis factor.TPSDKPVAHVVANPQAEGQLQWLNR.+3b4.light; Context=Skyline Template-MRM-TableWithRowCount=135-RowIndex-28, Property=CompoundIdProperty, Value = Tumor necrosis factor.TPSDKPVAHVVANPQAEGQLQWLNR.+3b5.light; Context=Skyline Template-MRM-TableWithRowCount=135-RowIndex-29, Property=CompoundIdProperty, Value = Tumor necrosis factor.TPSDKPVAHVVANPQAEGQLQWLNR.+3b6.light; Context=Skyline Template-MRM-TableWithRowCount=135-RowIndex-30, Property=CompoundIdProperty, Value = Tumor necrosis factor.TPSDKPVAHVVANPQAEGQLQWLNR.+3b7.light; Context=Skyline Template-MRM-TableWithRowCount=135-RowIndex-31, Property=CompoundIdProperty, Value = Tumor necrosis factor.TPSDKPVAHVVANPQAEGQLQWLNR.+3b8.light; Context=Skyline Template-MRM-TableWithRowCount=135-RowIndex-32, Property=CompoundIdProperty, Value = Tumor necrosis factor.TPSDKPVAHVVANPQAEGQLQWLNR.+3b9.light; Context=Skyline Template-MRM-TableWithRowCount=135-RowIndex-33, Property=CompoundIdProperty, Value = Tumor necrosis factor.TPSDKPVAHVVANPQAEGQLQWLNR.+3b10.light; Context=Skyline Template-MRM-TableWithRowCount=135-RowIndex-50, Property=CompoundIdProperty, Value = Tumor necrosis factor.DNQLVVPSEGLYLIYSQVLFK.+3y8.light; Context=Skyline Template-MRM-TableWithRowCount=135-RowIndex-51, Property=CompoundIdProperty, Value = Tumor necrosis factor.DNQLVVPSEGLYLIYSQVLFK.+3y7.light; Context=Skyline Template-MRM-TableWithRowCount=135-RowIndex-52, Property=CompoundIdProperty, Value = Tumor necrosis factor.DNQLVVPSEGLYLIYSQVLFK.+3y6.light; Context=Skyline Template-MRM-TableWithRowCount=135-RowIndex-53, Property=CompoundIdProperty, Value = Tumor necrosis factor.DNQLVVPSEGLYLIYSQVLFK.+3y5.light; Context=Skyline Template-MRM-TableWithRowCount=135-RowIndex-54, Property=CompoundIdProperty, Value = Tumor necrosis factor.DNQLVVPSEGLYLIYSQVLFK.+3y4.light; Context=Skyline Template-MRM-TableWithRowCount=135-RowIndex-55, Property=CompoundIdProperty, Value = Tumor necrosis factor.DNQLVVPSEGLYLIYSQVLFK.+3y3.light; Context=Skyline Template-MRM-TableWithRowCount=135-RowIndex-56, Property=CompoundIdProperty, Value = Tumor necrosis factor.DNQLVVPSEGLYLIYSQVLFK.+3y2.light; Context=Skyline Template-MRM-TableWithRowCount=135-RowIndex-57, Property=CompoundIdProperty, Value = Tumor necrosis factor.DNQLVVPSEGLYLIYSQVLFK.+3b2.light; Context=Skyline Template-MRM-TableWithRowCount=135-RowIndex-58, Property=CompoundIdProperty, Value = Tumor necrosis factor.DNQLVVPSEGLYLIYSQVLFK.+3b3.light; Context=Skyline Template-MRM-TableWithRowCount=135-RowIndex-59, Property=CompoundIdProperty, Value = Tumor necrosis factor.DNQLVVPSEGLYLIYSQVLFK.+3b4.light; Context=Skyline Template-MRM-TableWithRowCount=135-RowIndex-60, Property=CompoundIdProperty, Value = Tumor necrosis factor.DNQLVVPSEGLYLIYSQVLFK.+3b5.light; Context=Skyline Template-MRM-TableWithRowCount=135-RowIndex-61, Property=CompoundIdProperty, Value = Tumor necrosis factor.DNQLVVPSEGLYLIYSQVLFK.+3b6.light; Context=Skyline Template-MRM-TableWithRowCount=135-RowIndex-62, Property=CompoundIdProperty, Value = Tumor necrosis factor.DNQLVVPSEGLYLIYSQVLFK.+3b7.light; Context=Skyline Template-MRM-TableWithRowCount=135-RowIndex-63, Property=CompoundIdProperty, Value = Tumor necrosis factor.DNQLVVPSEGLYLIYSQVLFK.+3b8.light; Context=Skyline Template-MRM-TableWithRowCount=135-RowIndex-64, Property=CompoundIdProperty, Value = Tumor necrosis factor.DNQLVVPSEGLYLIYSQVLFK.+3b9.light; Context=Skyline Template-MRM-TableWithRowCount=135-RowIndex-65, Property=CompoundIdProperty, Value = Tumor necrosis factor.DNQLVVPSEGLYLIYSQVLFK.+3b10.light; Context=Skyline Template-MRM-TableWithRowCount=135-RowIndex-82, Property=CompoundIdProperty, Value = Tumor necrosis factor.GQGCPSTHVLLTHTISR.+2b10.light; Context=Skyline Template-MRM-TableWithRowCount=135-RowIndex-83, Property=CompoundIdProperty, Value = Tumor necrosis factor.GQGCPSTHVLLTHTISR.+2b11.light; Context=Skyline Template-MRM-TableWithRowCount=135-RowIndex-100, Property=CompoundIdProperty, Value = Tumor necrosis factor.GQGCPSTHVLLTHTISR.+3b10.light; Context=Skyline Template-MRM-TableWithRowCount=135-RowIndex-101, Property=CompoundIdProperty, Value = Tumor necrosis factor.GQGCPSTHVLLTHTISR.+3b11.light; Context=Skyline Template-MRM-TableWithRowCount=135-RowIndex-118, Property=CompoundIdProperty, Value = Tumor necrosis factor.ETPEGAEAKPWYEPIYLGGVFQLEK.+3y9.light; Context=Skyline Template-MRM-TableWithRowCount=135-RowIndex-119, Property=CompoundIdProperty, Value = Tumor necrosis factor.ETPEGAEAKPWYEPIYLGGVFQLEK.+3y8.light; Context=Skyline Template-MRM-TableWithRowCount=135-RowIndex-120, Property=CompoundIdProperty, Value = Tumor necrosis factor.ETPEGAEAKPWYEPIYLGGVFQLEK.+3y7.light; Context=Skyline Template-MRM-TableWithRowCount=135-RowIndex-121, Property=CompoundIdProperty, Value = Tumor necrosis factor.ETPEGAEAKPWYEPIYLGGVFQLEK.+3y6.light; Context=Skyline Template-MRM-TableWithRowCount=135-RowIndex-122, Property=CompoundIdProperty, Value = Tumor necrosis factor.ETPEGAEAKPWYEPIYLGGVFQLEK.+3y5.light; Context=Skyline Template-MRM-TableWithRowCount=135-RowIndex-123, Property=CompoundIdProperty, Value = Tumor necrosis factor.ETPEGAEAKPWYEPIYLGGVFQLEK.+3y4.light; Context=Skyline Template-MRM-TableWithRowCount=135-RowIndex-124, Property=CompoundIdProperty, Value = Tumor necrosis factor.ETPEGAEAKPWYEPIYLGGVFQLEK.+3y3.light; Context=Skyline Template-MRM-TableWithRowCount=135-RowIndex-125, Property=CompoundIdProperty, Value = Tumor necrosis factor.ETPEGAEAKPWYEPIYLGGVFQLEK.+3y2.light; Context=Skyline Template-MRM-TableWithRowCount=135-RowIndex-126, Property=CompoundIdProperty, Value = Tumor necrosis factor.ETPEGAEAKPWYEPIYLGGVFQLEK.+3b2.light; Context=Skyline Template-MRM-TableWithRowCount=135-RowIndex-127, Property=CompoundIdProperty, Value = Tumor necrosis factor.ETPEGAEAKPWYEPIYLGGVFQLEK.+3b3.light; Context=Skyline Template-MRM-TableWithRowCount=135-RowIndex-128, Property=CompoundIdProperty, Value = Tumor necrosis factor.ETPEGAEAKPWYEPIYLGGVFQLEK.+3b4.light; Context=Skyline Template-MRM-TableWithRowCount=135-RowIndex-129, Property=CompoundIdProperty, Value = Tumor necrosis factor.ETPEGAEAKPWYEPIYLGGVFQLEK.+3b5.light; Context=Skyline Template-MRM-TableWithRowCount=135-RowIndex-130, Property=CompoundIdProperty, Value = Tumor necrosis factor.ETPEGAEAKPWYEPIYLGGVFQLEK.+3b6.light; Context=Skyline Template-MRM-TableWithRowCount=135-RowIndex-131, Property=CompoundIdProperty, Value = Tumor necrosis factor.ETPEGAEAKPWYEPIYLGGVFQLEK.+3b7.light; Context=Skyline Template-MRM-TableWithRowCount=135-RowIndex-132, Property=CompoundIdProperty, Value = Tumor necrosis factor.ETPEGAEAKPWYEPIYLGGVFQLEK.+3b8.light; Context=Skyline Template-MRM-TableWithRowCount=135-RowIndex-133, Property=CompoundIdProperty, Value = Tumor necrosis factor.ETPEGAEAKPWYEPIYLGGVFQLEK.+3b9.light; Context=Skyline Template-MRM-TableWithRowCount=135-RowIndex-134, Property=CompoundIdProperty, Value = Tumor necrosis factor.ETPEGAEAKPWYEPIYLGGVFQLEK.+3b10.light.

Command-line: Method\AbSciex\SciexOS\BuildSciexMethod -d -m "D:\SCIEX OS Data\Dhaval_Proteins\Acquisition Methods\Skyline Template.msm" "D:\SCIEX OS Data\Dr German_ Inflammatory Proteins\Acquisition Methods\qx5pnf2o.wbd\transitions.txt"
Working directory: D:\SCIEX OS Data\Dr German_ Inflammatory Proteins\Acquisition Methods
   at pwiz.Common.SystemUtil.ProcessRunner.Run(ProcessStartInfo psi, String stdin, IProgressMonitor progress, IProgressStatus& status, TextWriter writer, ProcessPriorityClass priorityClass, Boolean forceTempfilesCleanup, Func`3 outputAndExitCodeAreGoodFunc, Boolean updateProgressPercentage) in C:\proj\skyline_23_1\pwiz_tools\Shared\Common\SystemUtil\ProcessRunner.cs:line 181
   at pwiz.Skyline.Util.Extensions.UtilProcess.RunProcess(ProcessStartInfo psi, String stdin, String messagePrefix, IProgressMonitor progress, IProgressStatus& status) in C:\proj\skyline_23_1\pwiz_tools\Skyline\Util\Extensions\UtilProcess.cs:line 45
   at pwiz.Skyline.Model.MethodExporter.ExportMethod(String exeName, List`1 argv, String fileName, String templateName, Dictionary`2 dictTranLists, IProgressMonitor progressMonitor) in C:\proj\skyline_23_1\pwiz_tools\Skyline\Model\Export.cs:line 4731
   at pwiz.Skyline.Model.SciexOsMethodExporter.ExportMethod(String fileName, String templateName, IProgressMonitor progressMonitor) in C:\proj\skyline_23_1\pwiz_tools\Skyline\Model\Export.cs:line 2864
   at pwiz.Skyline.Controls.LongWaitDlg.RunWork(Action`1 performWork) in C:\proj\skyline_23_1\pwiz_tools\Skyline\Controls\LongWaitDlg.cs:line 254
   --- End of inner exception stack trace ---
   at pwiz.Skyline.Util.Helpers.WrapAndThrowException(Exception x) in C:\proj\skyline_23_1\pwiz_tools\Skyline\Util\Util.cs:line 1922
   at pwiz.Skyline.Controls.LongWaitDlg.PerformWork(Control parent, Int32 delayMillis, Action`1 performWork) in C:\proj\skyline_23_1\pwiz_tools\Skyline\Controls\LongWaitDlg.cs:line 202
   at pwiz.Skyline.Controls.LongWaitDlg.PerformWork(Control parent, Int32 delayMillis, Action`1 performWork) in C:\proj\skyline_23_1\pwiz_tools\Skyline\Controls\LongWaitDlg.cs:line 140
   at pwiz.Skyline.FileUI.ExportDlgProperties.PerformLongExport(Action`1 performExport) in C:\proj\skyline_23_1\pwiz_tools\Skyline\FileUI\ExportMethodDlg.cs:line 2313
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