Use one compound to calibrate different molecules in mixture

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Use one compound to calibrate different molecules in mixture NewUser  2023-09-07 11:29
 

I am trying to generate a quantification method on skyline-daily where I have a set of calibration standards (of compound A) and then use this calibration curve to quantify two other compounds (A, B and C) in my actual samples. Is that possible using skyline? I tried using surrogate IS but that doesn't give me the curve correctly.

Note: Compounds B and C are not present in the calibration standards.