Hello Skyline Team,
I am looking to put together a spectral library using an .ssl file, and I was wondering if it is possible to create a spectral library using this file with MS2 scans that did not match from a database search? For example, instead of including the peptide sequence, when creating the spectral library, we can use different names, like this:
file scan charge sequence
demo.ms2 8 3 PrecursorID1
demo.ms2 1806 2 LAESITIEQGK
demo.ms2 2572 2 PrecursorID2
demo.ms2 3088 2 TTAGAVEATSEITEGK
demo.ms2 3266 2 DC[+57.0]EEVGADSNEGGEEEGEEC[+57.0]
demo.ms2 9734 3 IWELEFPEEAADFQQQPVNAQ[-17.0]PQN
demo.ms2 20919 3 VHINIVVIGHVDSGK
../elsewhere/spec.mzXML 00497 2 LKEPAQNTADNAK
../elsewhere/spec.mzXML 00680 2 ALEGPGPGEDAAHSENNPPR
../elsewhere/spec.mzXML 00965 2 FFSHEAEQK
../elsewhere/spec.mzXML 01114 2 C[+57.0]GPSQPL
In addition, can we only add the retention time column for this file and not add the score columns?
Thank you.
David