Hi,

Skyline has been a great tool for my lab to generate peak areas normalised to IS or surrogate standard, however I was excited by the ability to generate multiple calibration curves and thus quantification using the 'Concentration Multiplier' as outlined at https://skyline.ms/wiki/home/software/Skyline/page.view?name=tutorial_hi_res_metabolomics.

I am a little confused as to how to complete the workflow, since the Concentration Multiplier only seems visible in peptide mode (not molecules).

Perhaps I should load my transition lists as peptides, however this seems tricky due to the requiements of specific information which does not exist for small molecules.

I am using skyline version 22.2.0.52.7.

Any information would be appreciated.

Best wishes,
Matt