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Complete peaks not displayed in skyline but see in Analyst

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Complete peaks not displayed in skyline but see in Analyst kansalm  2023-06-28 14:06
 

Hi, My peaks (lipids) don't show in skyline but I see them in raw data file. Also some other peaks (lipids) are cutting off in skyline but full peak in the raw file. Please see the attached

Thanks,
Monika
 
 
Nick Shulman responded:  2023-06-28 14:20
Can you send us your Skyline document and your .wiff2 file and the .wiff.scan file that goes with that .wiff2 (also, if you happen to have a .wiff file, you can send that too).

In Skyline you can use the menu item:
File > Share
to create a .zip file containing your Skyline document and supporting files including extracted chromatograms.

Files which are less than 50MB can be attached to this support request.

If your files are bigger than that you can zip them all up and upload them here:
https://skyline.ms/files.url

-- Nick
 
kansalm responded:  2023-06-28 14:50
Please see attached file for tPGEM
 
kansalm responded:  2023-06-28 14:53
Please see file for extracted ion in OS software
 
Nick Shulman responded:  2023-06-28 15:05
Can you send me the files "20230601_Lipids_SS-3.wiff" and "20230601_Lipids_SS-3.wiff.scan" (if it exists)?
If those files are less than 50MB you can attach them to this support request. You can upload larger files here:
https://skyline.ms/files.url
-- Nick