Hi all,
I want to build a peptide spectral library by using .raw file,and then when I use the DDA Search for MS1 Filtering of the Skyline, the results show that the search failed,so I convert the format of my fasta files to .mz5,because I find the file format of tutorials is .mz5,then the results show “No spectra were found for the new library”.
Can anyone tell me how to fix this issue?