|Error: No spectra were found for the new library||Jess||2023-04-03|
I am doing proteomic work and wanting to build a peptide spectral library using .dat files from Mascot. My software and system specifications are:
Skyline (64-bit) 126.96.36.1991 (28f9b9301)
I generated mgf files from MZML files using MSConvertGUI (64-bit). My Mascot peptide searches have thousands of results. When I go into Skyline to build a spectral library, I go Settings>Peptide Settings>Library>Build. I choose my output path, and add my .dat files. Skyline then returns the error message "ERROR: no spectra were found for the new library".
I have attached 3 screenshots for 3 different instances where I received the error messages, described below:
Can anyone tell me how to fix this issue? Is it a problem with my DAT file, or a problem with how I am generating my spectral library in Skyline?