|Instructions for normalizing with TIC||dave lee-2||2023-02-02|
I have a PRM experiment where I am trying to normalize each sample according to their respective TIC.
However, I have come across a couple of issues -> The "Explicit Global Standard Area" is under the "calculated" tab rather than "editable" in the document settings so I can't seem to copy and paste.
Ultimately, what I would like is when I view the peak areas of my peptides (with respect to the other replicates); I would like to normalize against the TIC -> Currently, the only options available are "Total", "Maximum" and "None"
Is there any resource that provides step-by-step instructions in what I am attempting to do?
The version of Skyline I'm using is: