|Fitting cross-linked peptides to an iRT scale||Juan C. Rojas E.||2023-01-16|
I am trying to fit some cross-linked peptides to an iRT scale (i.e. calculator) generated from endogenous peptides. Unfortunately the column had to be changed, resulting in different retention times. However, I am trying to compensate for this using the background proteome to define some iRTs.
Skyline allows me to add the peptides within the "Edit iRT Calculator" by clicking "Add Results..." but when I try to click OK I am getting a error message (attached).
You will know best, but to me it seems is that here Skyline is not recognizing cross-link modifications?
Any suggestions for a workaround or is it something that has to be patched?
As always, thanks for your time and help.