Error retrieving chromatogram "UV 1"

support
Error retrieving chromatogram "UV 1" licky lck  2022-11-01
 

Dear Skyline team,

The samples run from Our Q Eaxctive had a hard time recording the UV data, so we could not retrieve the UV data after the sequence run. So when I am trying to import the raw file into skyline, this error message pops out "Failed importing results file 'XXXX.raw'.
[ChromatogramList_Thermo::chromatogram()] Error retrieving chromatogram "UV 1": [RawFileImpl::getChromatogramData()] Unknown UV/PDA packet type". (The full error message can be found below)

Do you have any suggestions to recover the data from my raw file, or can skyline ignores this UV signal but import my MS data?

Full error message:
Failed importing results file 'XXXX.raw'.
[ChromatogramList_Thermo::chromatogram()] Error retrieving chromatogram "UV 1": [RawFileImpl::getChromatogramData()] Unknown UV/PDA packet type
pwiz.Skyline.Model.Results.ChromCacheBuildException: Failed importing results file 'XXXX.raw'.
[ChromatogramList_Thermo::chromatogram()] Error retrieving chromatogram "UV 1": [RawFileImpl::getChromatogramData()] Unknown UV/PDA packet type ---> System.Exception: [ChromatogramList_Thermo::chromatogram()] Error retrieving chromatogram "UV 1": [RawFileImpl::getChromatogramData()] Unknown UV/PDA packet type
at pwiz.XX.msdata.ChromatogramList.chromatogram(Int32 index, DetailLevel detailLevel)
at pwiz.ProteowizardWrapper.MsDataFileImpl.GetQcTraces() in C:\proj\skyline_22_2\pwiz_tools\Shared\ProteowizardWrapper\MsDataFileImpl.cs:line 846
at pwiz.Skyline.Model.Results.GlobalChromatogramExtractor..ctor(MsDataFileImpl dataFile) in C:\proj\skyline_22_2\pwiz_tools\Skyline\Model\Results\ChromDataProvider.cs:line 129
at pwiz.Skyline.Model.Results.SpectraChromDataProvider..ctor(MsDataFileImpl dataFile, ChromFileInfo fileInfo, SrmDocument document, IRetentionTimePredictor retentionTimePredictor, String cachePath, IProgressStatus status, Int32 startPercent, Int32 endPercent, IProgressMonitor loader) in C:\proj\skyline_22_2\pwiz_tools\Skyline\Model\Results\SpectraChromDataProvider.cs:line 87
at pwiz.Skyline.Model.Results.ChromCacheBuilder.CreateSpectraChromProvider(MsDataFileImpl dataFile, ChromFileInfo fileInfo) in C:\proj\skyline_22_2\pwiz_tools\Skyline\Model\Results\ChromCacheBuilder.cs:line 1306
at pwiz.Skyline.Model.Results.ChromCacheBuilder.BuildCache() in C:\proj\skyline_22_2\pwiz_tools\Skyline\Model\Results\ChromCacheBuilder.cs:line 244

 
 
Brian Pratt responded:  2022-11-01

You could try converting to mzML with msconvert, but it may well have the same issue.

We'd be happy to look at the file if you find yourself stuck.

Thanks for using the Skyline support board,

Brian Pratt