Hi Rita,
I have just uploaded "2019-08 Brixen Summerschool files.zip" to the Skyline support board file folder:
https://skyline.ms/files.url
This contains a more basic PRM tutorial than the one we used during the course. It still uses a spectral library and a protein sequence. Though, you can substitute Edit > Insert > Peptides where it uses Edit > Insert > Proteins for your peptide of interest. We used Edit > Insert > Peptides multiple times during Skyline-Online: Session 1. For the Absotlute Quant tutorial, we used it to add just a single peptide with no protein sequence information, as you may want to do here.
Since, your PowerPoint slides mention wanting to do this for a calibration curve, you will want to review the Absolute Quantification tutorial we covered during the session. It is actually very similar to what you seem to want to do, since it is just one peptide with a heavy standard reference. It is, however, an experiment performed with SRM on a triple-quadrupole.
So, I hope the extra simplified PRM tutorial for an Orbitrap will be helpful. If after reviewing the new tutorial, the PRM Orbitrap tutorial from Session 1, and the Absolute Quantification tutorial, you still feel stuck, post back to this thread and we will arrange for some kind of Zoom review to get you unstuck.
Good luck. Thanks for posting to the support board and for your time and attention during Session 1.
--Brendan