I am trying to build a spectral library with .msf files but it keeps showing an error: "This file does not contain q-values. You can set a cut-off score of 0 in order to build a library from it"
However I cannot find where I can set this cut-off score. Could you please help me find?! I am using Skyline 22.214.171.124; in older versions there was a small window where we could set the cut-off score...
Kaipo Tamura responded:
After you add the .msf as an input file, you can set the cut-off score by editing its last column in the grid.