PRM or DIA analysis for large number of proteins

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PRM or DIA analysis for large number of proteins asamaras  2022-08-02
 

Hi all,

I am during an experiment with target proteomics with non labelled peptides. My targets are fungal proteins in infected plant tissue. The goal of my study is to detect and then to quantify the proteins. I am trying to find the most sensitive way to detect them. I try to determine the retention time of the proteins of interest (350), but as I expecten the range of the retention time is really big, so I don't know if something like this can load it in the instruments. So, my question is , do you beleive that there is a limit of proteins when you want to predict retention time using PRM?? The other alternative is DIA running method. Does anyone has the same issue?

Thank you in advance for your response,
Best
Tasos