BlibBuild not reading Thermo raw files | lauri.peil | 2013-09-20 02:04 | |||||||||||||||||||||||||||||||||||||||
Hi, I have run into a problem with generating spectral libraries from .ssl files. I have a self-generated .ssl file according to instructions in https://skyline.gs.washington.edu/labkey/wiki/home/software/BiblioSpec/page.view?name=BiblioSpec%20input%20and%20output%20file%20formats and would like to use it in Skyline. However, when I try to build spectral library from that .ssl file, Skyline reports an error: ERROR: [MSDataFile::readFile()] Unsupported file format.. ERROR: PwizReader could not parse my_raw-file.raw MSFileReader is installed and Skyline works just fine with Thermo files for other spectral libraries so this can't be the issue. Furthermore, I tried compiling BiblioSpec fresh and got exactly the same error when using command-line BlibBuild (vendor libraries were included and build process found them, for example msconvert worked fine with .raw files). There seems to be an 'old' v2.0.0 pre-compiled BiblioSpec available (http://proteome.gs.washington.edu/software/bibliospec/documentation/download.html) which requires slightly different .ssl input but otherwise works fine, can find MSFileReader and use it for reading in .raw files. Looks to me as current BiblioSpec/BlibBuild has a bug somewhere so that it does not link with MSFileReader libraries and therefore can't parse .raw files, unless I have done something completely wrong. Any ideas? Thanks a lot, Lauri |
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