Zeno MRM Wiff 2 file

Zeno MRM Wiff 2 file bkr  2022-05-04


I am currently working on Zeno MRM data and I am not able to import wiff2 data into skyline.

I get the following error

"At 13:10:
Failed importing results file 'F:\20220504\GA_Zenooff_q1_open_50ms_MRMHR_01.wiff2'.
[WiffFile2Impl::ctor()] Object reference not set to an instance of an object."

Kindly help me with this.

It would be also great if you can suggest transition settings for this type of data


Nick Shulman responded:  2022-05-04
I believe the only wiff2 files that Skyline (and ProteoWizard) can handle are those from the x500 machine. If you have a different type of mass spectrometer then you need to use the .wiff file instead of the .wiff2.

If that does not fix your problem then you can send us your files and we can take a look. I imagine we would need your .wiff, .wiff2 and .wiff.scan files.
Files which are less than 50MB can be attached to this support request. You can upload larger files here:
-- Nick