| How to eaxtract result from two different product ion experiement from QTOF, same precrusor, same product, different collision energy |
karin yanagi |
2022-04-16 14:36 |
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This is a run to look at free fatty acid and I have two collision energy set for each mass (for example 253.22 m/z for palmitiec acid, i setted CE as -25 and -15) I want to look at the product ion as same mass as the prevcursor in different collision enegy. They should have different intensity, but no matter how i define the collision energy in the transition list. It seems to lead me to same chromtagram.
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| Nick Shulman responded: |
2022-04-16 15:28 |
| Title: How to extract result from two different product ion experiement from QTOF, same precrusor, same product, different collision energy |
When Skyline is reading SRM chromatograms Skyline does not pay attention to the collision energy. You just get the first chromatogram that most closely matches the Q1 and Q3 m/z's
If you want to have two sets of chromatograms with different collision energies, I would recommend changing the Q1 value by a small amount such as .01 and also have two precursors in your Skyline document whose m/z's match that.
--Nick |
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| karin yanagi responded: |
2022-04-17 01:53 |
That clarify my question, I will do that next time. Thanks for the quick respond! |
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StdA_20um.wiff