issue with chromatogram information unavailable from DDAsearchMS1

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issue with chromatogram information unavailable from DDAsearchMS1 diletta piana  2022-04-12
 

Hi, I am Diletta Piana a PhD student and I did a DDAsearch to find a specific protein and transition.
I don't know why I have identified the protein and it shows me the peptide and precursor but the chromatogram information is unavailable.
Do you know what the answer to this problem is?
How can I see the chromatogram information of this peptide?
Do you suggest me to do other search ?
Looking forward to your kind reply,

Kind regards,

Diletta Piana

 
 
Brian Pratt responded:  2022-04-12

Hi Diletta,

This will be easiest to diagnose if you can provide your Skyline document (use Skyline's File>Share menu item) and the mass spec data file in question. You can upload those to http://skyline.ms/files.url, or someplace more private by arrangement.

Thanks for using the Skyline support board,

Brian Pratt