SureQuant Tutorial for Custom Method

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SureQuant Tutorial for Custom Method comtewal  2022-03-31
 

Dear Skyline Developers,

Would it be possible to create a generic tutorial to use when acquiring data on a Thermo instrument using a SureQuant custom method (as opposed to using the commercial kits of reagents/peptides) supplied by Thermo.

I have been trying to create a Skyline method to quantify our peptides of interest using Skyline v. 21.2.0.425 but the transitions for my peptides (heavy and light versions) are importing but I am not able to visualize them.

Thank you so much,
Susana Comte-Walters
MS Redox Core Facility
Medical University of SC
comtewal@musc.edu

 
 
Nick Shulman responded:  2022-05-09
Sorry for the slow response.
We have found a SureQuant tutorial which was used in a course last year. You can see the tutorial here:
https://skyline.ms/wiki/home/software/Skyline/page.view?name=TriggeredAcquisition

I hope this helps. Let me know if you still have any questions.
-- Nick