SureQuant Integration Errors

SureQuant Integration Errors romeally  2022-03-10

I am using SureQuant and I sometimes get errors integrating the peaks with a line being drawn across the entire chromatogram. It seems like it accidentally triggers early and uses that point sometimes 10s of minutes early and extrapolates to the actual peak. I am attaching an image of the full chromatogram. It seems like if the software had a feature that would only integrate if there are at least 3 consecutive scans or something like that this first erroneous point would not be incorporated. Has anyone encountered this error and if so is there a workaround? Thank you for your help!

Nick Shulman responded:  2022-03-10
When you have "Triggered chromatogram acquisition" checked at "Settings > Transition Settings > Instrument", Skyline will try to detect where the gaps in the chromatogram are. This feature is probably working correctly in your document, but I think the problem is that the graph that you see when you have multiple peptides selected does not know anything about this feature.

If you were to select one peptide in the Targets tree, then the graph that you see would not have those long horizontal lines. When "Triggered chromatogram acquisition" is selected, the chromatogram line is supposed to have breaks in it, with blank space in the regions where no light MS2 spectra were acquired.

I will try to fix the multi-peptide graph display so that it works correctly with SureQuant data.

It is possible that there is something else going wrong. If you would like, you can send us your Skyline document.
In Skyline you can use the menu item:
File > Share
to create a .zip file containing your Skyline document and supporting files including extracted chromatograms.
If that .zip file is less than 50MB you can attach it to this support request. You can upload larger files here:
-- Nick