Sciex 7500 CE/DP optimization, which equation to use?

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Sciex 7500 CE/DP optimization, which equation to use? Erik  2022-02-24
 

Hi Skyline team,

I would like to perform some CE (and try some DP) optimizations on phosphopeptides on a Sciex7500.
For the Altis there was a single option. However for Sciex I see multiple options:
For CE:

  • SCIEX
  • QTRAP 6500
  • ABI 5500 Q trap PO4

For DP:

  • SCIEX
  • Static_PO4

I'm a bit confused which equation to use in my case; a MS model that is not listed and investigating PO4 peptides. What are the different equations based on ? And which one would you recommend?
Also the CE values can change a lot depending on the equation (see attachment).

Greetings,
Erik

 
 
Erik responded:  2022-02-25

I got word from a Sciex application specialist that the QTRAP 6500 gives good first estimates for the right CE on the 7500.