Dear Skyline team,
I’ve recently tried to import a transition list containing C12 and fully C13 labelled precursors with [M+H]+ and [M-H]- adducts and noticed that for small compounds containing only 2 carbon atoms the heavy [M-H]- precursors are not represented. I assume the problem might originate from the similar mass to charge ratios for light positive and heavy negative molecules. Do you have any idea how to deal with this issue? I’m using Windows 10 and Skyline (64-bit) 126.96.36.1999 (2efacf038) and my transition list for Taurin looks like this:
Molecule List Name Precursor Name Precursor Formula Precursor Adduct Precursor Charge Label Type
compound_of_interest Taurin C2H7NO3S [M+H]+ 1 light
compound_of_interest Taurin C'2H7NO3S [M+H]+ 1 heavy
compound_of_interest Taurin C2H7NO3S [M-H]- -1 light
compound_of_interest Taurin C'2H7NO3S [M-H]- -1 heavy
Looking forward to hearing from you!