iRT peptides/protein as global standard | Juan C. Rojas E. | 2022-01-06 01:34 | |||||||||||||||
Hi all, I spiked a protein to my sample before performing the digestion. Then I used the peptides of this protein to define the custom iRT peptides. The main purpose of the protein spike was to control variance of the digestion procedure so I would like to set it as a global standard. However, when I tried to set the peptides of this protein as a global standard the option is greyed out (image attached). What is the reason behind this? Besides removing the iRT predictor (and with it the annotated iRT peptides) what else could I do to consider these peptides as global standards? Would you suggest I use all the peptides of the protein or just a few intense ones? If the second, how many? As always, thank you for your help and time. PS: I am using Skyline 21.2.0.369 |
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