|Settings questions arising from Webinar 21: PASEF||paga||2021-12-13|
I've been going through the recently uploaded tutorial on DIA-PASEF, and I have the following doubts I would love to solve.
1.- I understand why they select only precursors with charges 2 and 3, but what I don't understand is why they decide not to look at the precursors (MS1 spectra). They decide to avoid using MS1-filtering, and decided to only use MS/MS filtering with a Centroid Precursor mass analyzer. Why would be a good idea to dispense on valuable precursor information which is used to determine transition quality (idotp)?
2.- On the same line, why is Centroid better than TOF for PASEF?
3.- When selecting MS/MS filtering with TOF Product mass analyzer, the default resolving power is 30.000. On the PASEF tutoriual, the chocen Centroid option was paird with a value of 30 for resolving power. What is the source of this huge difference? Is there a way I can check the resolving power of my DIA files in case I wasn't the one performing them?
Thanks a lot for the support, Skyline team!