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normalization using MSstats

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normalization using MSstats Sam110  2021-11-16 12:57
 

Hello,

I wanted to use the data normalization function in MSstats, however I am getting an error :
"Error in SkylinetoMSstatsFormat(raw, removeProtein_with1Feature = TRUE, :
** Please check annotation. Each MS run can't have multiple conditions or BioReplicates.
Can't finish analysis."

I have unique names and bioreplicate numbers for each file so I'm sure where I am going wrong.

Thank you
 
 
Nick Shulman responded:  2021-11-16 13:59
This error happens if you have imported the same result file more than once, so that it belongs to more than one Replicate.

You should go to:
Edit > Manage Results
and remove some of the replicates so that any particular result file only belongs to a single replicate.

Here is another support request where someone ran into the same problem:
https://skyline.ms/announcements/home/support/thread.view?rowId=52004

-- Nick