Skyline cannot import result file

support
Skyline cannot import result file guo xue  2021-09-27
 

Hi, I installed skyline on my laptop. but I am not able to import raw files. the error info is pasted below. I tried to copy all the raw files to local drive it also failed. Can you please help with this issue?

At 3:23 pm:
Failed importing results file 'Y:\Collaboration_Services\Raw_files_FPL\Fusion Lumos\2021\Guo Xue\Ub\210923_Whole_titration_A549_Raw\210923_GX_Ub_A549_W_M2_100pg_spike.raw'.

pwiz.Skyline.Model.Results.ChromCacheBuildException: Failed importing results file 'Y:\Collaboration_Services\Raw_files_FPL\Fusion Lumos\2021\Guo Xue\Ub\210923_Whole_titration_A549_Raw\210923_GX_Ub_A549_W_M2_100pg_spike.raw'.
---> pwiz.Skyline.Util.AssumptionException
at pwiz.Skyline.Util.Assume.Fail(String error) in C:\proj\skyline_21_1_x64\pwiz_tools\Skyline\Util\Util.cs:line 2055
at pwiz.Skyline.Model.Results.SpectrumFilterPair..ctor(PrecursorTextId precursorTextId, Color peptideColor, Int32 id, Nullable1 minTime, Nullable1 maxTime, Boolean highAccQ1, Boolean highAccQ3) in C:\proj\skyline_21_1_x64\pwiz_tools\Skyline\Model\Results\SpectrumFilterPair.cs:line 57
at pwiz.Skyline.Model.Results.SpectrumFilter..ctor(SrmDocument document, MsDataFileUri msDataFileUri, IFilterInstrumentInfo instrumentInfo, Nullable`1 maxObservedIonMobilityValue, IRet/entionTimePredictor retentionTimePredictor, Boolean firstPass, GlobalChromatogramExtractor gce) in C:\proj\skyline_21_1_x64\pwiz_tools\Skyline\Model\Results\SpectrumFilter.cs:line 267
at pwiz.Skyline.Model.Results.SpectraChromDataProvider..ctor(MsDataFileImpl dataFile, ChromFileInfo fileInfo, SrmDocument document, IRetentionTimePredictor retentionTimePredictor, String cachePath, IProgressStatus status, Int32 startPercent, Int32 endPercent, IProgressMonitor loader) in C:\proj\skyline_21_1_x64\pwiz_tools\Skyline\Model\Results\SpectraChromDataProvider.cs:line 121
at pwiz.Skyline.Model.Results.ChromCacheBuilder.CreateSpectraChromProvider(MsDataFileImpl dataFile, ChromFileInfo fileInfo) in C:\proj\skyline_21_1_x64\pwiz_tools\Skyline\Model\Results\ChromCacheBuilder.cs:line 1264
at pwiz.Skyline.Model.Results.ChromCacheBuilder.BuildCache() in C:\proj\skyline_21_1_x64\pwiz_tools\Skyline\Model\Results\ChromCacheBuilder.cs:line 233
--- End of inner exception stack trace ---

 
 
Nick Shulman responded:  2021-09-27
This is caused by a bug in Skyline. This happens if you specified one but not both values for "Min time" and "Max time" on the Instrument tab of the Transition Settings dialog.

To fix this, you should go to:
Settings > Transition Settings > Instrument
and fill in a value for Min time and Max time.

This bug will be fixed in the next update of Skyline-Daily which will probably be released in a few weeks.
-- Nick