Proteome Discoverer mzid file could not be imported
mmr6q
2021-09-20 08:06
The error message stated that the mzid file contains an unsupported score type.
Please advise.
Thank you.
Kaipo Tamura responded:
2021-09-20 10:19
Does your Proteome Discoverer workflow include Percolator? Skyline requires probability scores (e.g. q-values from Percolator) in results files when building spectral libraries.
You may be able to build a spectral library from Proteome Discoverer results without q-values by setting the cut-off score to 0, but this is not recommended.
mmr6q responded:
2021-09-21 06:01
Nick,
I am trying to use a DDA raw file from the analysis of the PRTC standard. Percolator will not work because there are too few peptides. If I set the Skyline cut-off score to 0, I obtain the same error message of an unsupported score type.
Mark
mmr6q responded:
2021-09-21 10:25
Nick,
I used the PD msf file instead of the mzid file, and the import worked correctly (without using Percolator).
Thank you.
Mark