Error Importing Transition List: # of fields not picking up?

Error Importing Transition List: # of fields not picking up? jana carpenter7  2021-08-25

I'm hoping to see if there is a solution to a formatting issue with importing a CSV transition list for small molecules.

I will attach my working list to this message. The error messages that I've been receiving are:

"Failed reading the file...Line 'Organic Acid and Derivative' has 1 fields when 5 expected."

"Failed reading the file...Data not found in first column"

I am trying to calculate masses of metabolites with corresponding adducts, and have been able to in the past (for isotopically labeled molecules), but I'm not sure what has changed about how I'm formatting the document. Please let me know if there is a way to fix this issue. Thanks!


Brian Pratt responded:  2021-08-25

Hi Jana,

There are a couple of strange things here that are confusing Skyline.

Line 1 - there are a couple of tab characters in "MoleculeGroup ", that can't be helping.
Line 22 - there's a line break in "Organic Acid and Derivative _Aliphatic Acyclic Compound" (and others following), that's not supported.

Try tidying those up and let me know how it goes.

Thanks for using the Skyline support board!

Brian Pratt