no calibration curve shown for one analyte but others have it

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no calibration curve shown for one analyte but others have it laura.dubois  2021-08-16
 

Dear Skyline team,

One of my calibration curves won't show up (error is "The selected replicate has missing or truncated transitions") but all other calibration curves for all other analytes look fine. Any idea what is going on? Can I send you a shared file privately to see if you can pinpoint the problem file?

Thank you!
Laura Dubois

 
 
Nick Shulman responded:  2021-08-16
I will email you directly so you can send us a copy of your Skyline document.
-- Nick
 
Nick Shulman responded:  2021-08-16
Thanks for sending that Skyline document.

In your document, the light and heavy precursors have a different number of transitions, and the transitions have not been given any Product Names. When the transitions do not have names, Skyline matches up the light and heavy transitions based on their position under their parent precursor. This can lead to (for some) unexpected results, especially if a different pattern of transitions in the heavy and light precursor have been marked as non-quantitative.

If you would like Skyline to calculate ratios by summing up the light and heavy quantitative peak areas, without trying to match up the light and heavy transitions with each other, you can check the "Simple precursor ratios" checkbox at "Settings > Molecule Settings > Quantification".

-- Nick