|extract chromatograms from tims TOF||tleonova||2021-06-18|
Dear Skyline team support,
I am quite new to Skyline and I try to use it for MS1 filtering and spectra library building.
I have data acquired on Bruker tims TOF. I used MGF files created by Bruker software for the search in Proteome Discoverer 2.2.
I want to import peptide search from Proteome Discoverer (.msf). But I got stuck at the Extract Chromatograms window. The MGF files were missing.
I found that you already answered to the similar request. I realised that I have to add RAW files for chromatogram extraction. In my case RAW files are .d folders. But when I try to locate these files by clicking on the Find button, navigating to the RAW file location, and clicking these .d folders, nothing happens.
Am I doing something wrong?
Thanks in advance for your help!