|in silico trypsin digestion from a FASTA file||nicolas drouin||2021-05-27|
Dear Skyline team,
I encounter a problem with the in silico digestion of my FASTA file with trypsin (KR/P).
Another problem I see is the count of the residue. Indeed, this first peptide K.RFDNPVLPFNDGVYFASTEK. Indeed, according to the fast file, the residue R is located in position 78 but skyline indicated in position 77.
I'm running the last version of skyline-daily.
Please find enclosed the skyline file I'm using.
Many thanks in advance.