Can you expand on that a bit?

If you're asking whether Skyline can attempt to identify an unknown molecule by searching spctral libraries, the answer is no, Skyline is for targeted use. The assumption is that you already have some idea of what's in your sample. So we're normally downstream from that kind of search tool.

Thanks for using the Skyline support board!

Brian Pratt

(That said, Skyline does allow you to define a molecule and its fragments purely in terms of masses, so you can certainly work with unknowns in Skyline - it's just not the right tool with which to automatically ID them.)