Mismatch in PPP for experiment file viewed in Mass Lynx

support
Mismatch in PPP for experiment file viewed in Mass Lynx kartik kundgol  2021-05-13
 

Hello Support Team,

We are using SkylineRunner.exe to create Experiment files and form which we create Sample list which is summited to the Mass Lynx as batch.
We use Xevo TQ S instrument with SCN 1001.
Now when Method editor in MassLynx opens the experiment files, PPP(point per peak) value is less than expected and this results in less number of methods to be created.
Does SkylineRunner.exe does any optimization for give PPP value ??

Please suggest.
Thanks
Regards
Kartik

 
 
Nick Shulman responded:  2021-05-13
I am not sure I understand your question, but it might be easier to answer it if we could see your files.

In Skyline you can use the menu item:
File > Share
to create a .zip file containing your Skyline document and supporting files including extracted chromatograms.

If that .zip file is less than 50MB you can attach it to this support request. Otherwise, you can upload it here:
https://skyline.ms/files.url

It sounds like you are saying that you are using the "--export-method" command line argument to tell SkylineRunner to export an instrument method. Please let us know what your entire command line is.

Have you tried exporting the method using the regular Skyline graphical user interface (menu item "File > Export > Method")? If so, is the method that you get from the command line different than what you get using Skyline menus?
-- Nick